p130 Cas (phospho Tyr410) Polyclonal Antibody

    • 货号:YP0605
    • 应用:WB,IHC-p,IF(paraffin section),ELISA
    • 种属:Human,Mouse,Rat
      • 基因名称:
      • BCAR1
      • 蛋白名称:
      • Breast cancer anti-estrogen resistance protein 1
      • Human Gene Id:
      • 9564
      • Human Swiss Prot No:
      • P56945
      • Mouse Swiss Prot No:
      • Q61140
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr410. AA range:376-425
      • 特异性:
      • Phospho-p130 Cas (Y410) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y410.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Rabbit
      • 稀释:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
      • 分子量:
      • 93362
      • 实测条带:
      • 93
      • 背景:
      • BCAR1, Cas family scaffolding protein(BCAR1) Homo sapiens BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],
      • 功能:
      • domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphoryl
      • 细胞定位:
      • ruffle,nucleolus,cytoplasm,cytosol,plasma membrane,focal adhesion,actin cytoskeleton,lamellipodium,
      • 组织表达:
      • Caudate nucleus,Cerebellum,Cornea,Epithelium,Kidney,Lung,Mammary ca
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr410) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from NIH/3T3 cells, using p130 Cas (Phospho-Tyr410) Antibody. The lane on the right is blocked with the phospho peptide.