BAM32 (phospho Tyr139) Polyclonal Antibody

    • 货号:YP0549
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • DAPP1
      • 简介:
      • >>B cell receptor signaling pathway
      • 基因名称:
      • DAPP1
      • 蛋白名称:
      • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
      • Human Swiss Prot No:
      • Q9UN19
      • Mouse Swiss Prot No:
      • Q9QXT1
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human DAPP1 around the phosphorylation site of Tyr139. AA range:105-154
      • 特异性:
      • Phospho-BAM32 (Y139) Polyclonal Antibody detects endogenous levels of BAM32 protein only when phosphorylated at Y139.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • DAPP1;BAM32;HSPC066;Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide;hDAPP1;B lymphocyte adapter protein Bam32;B-cell adapter molecule of 32 kDa
      • 实测条带:
      • 32kD
      • 背景:
      • function:May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.,induction:Upon B-cell activation.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH2 domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation.,subunit:Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.,tissue specificity:Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.,
      • 功能:
      • function:May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.,induction:Upon B-cell activation.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH2 domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation.,subunit:Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.,tissue specificity:Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.,
      • 细胞定位:
      • Cytoplasm . Membrane ; Peripheral membrane protein . Membrane-associated after cell stimulation leading to its translocation.
      • 组织表达:
      • Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
      • 产品图片
      • Western Blot analysis of 293 cells using Phospho-BAM32 (Y139) Polyclonal Antibody
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPP1 (Phospho-Tyr139) Antibody
      • Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2', using DAPP1 (Phospho-Tyr139) Antibody. The lane on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).