Tak1 (phospho Thr187) Polyclonal Antibody

    • 货号:YP0424
    • 应用:WB,IHC-p,IF(paraffin section),ELISA
    • 种属:Human,Mouse,Rat
      • 基因名称:
      • MAP3K7
      • 蛋白名称:
      • Mitogen-activated protein kinase kinase kinase 7
      • Human Gene Id:
      • 6885
      • Human Swiss Prot No:
      • O43318
      • Mouse Swiss Prot No:
      • Q62073
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human MAP3K7 around the phosphorylation site of Thr187. AA range:161-210
      • 特异性:
      • Phospho-Tak1 (T187) Polyclonal Antibody detects endogenous levels of Tak1 protein only when phosphorylated at T187.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Rabbit
      • 稀释:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • MAP3K7; TAK1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1
      • 分子量:
      • 67196
      • 实测条带:
      • 60
      • 背景:
      • mitogen-activated protein kinase kinase kinase 7(MAP3K7) Homo sapiens The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Stimulates NF-kappa-B activation and the p38 MAPK pathway.,PTM:Association with MAP3K7IP1 promotes autophosphorylation and subsequent activation. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with MAP3K7IP1 and MAP3K7IP2. Interacts with PPM1L. Interaction with PP2A and PPP6C leads to its' repressed activity.,
      • 细胞定位:
      • nucleus,Ada2/Gcn5/Ada3 transcription activator complex,cytoplasm,cytosol,plasma membrane,IkappaB kinase complex,endosome membrane,
      • 组织表达:
      • Epithelium,Lung,Platelet,Uterus,

      Lei, WL., Han, F., Hu, MW. et al. Protein phosphatase 6 is a key factor regulating spermatogenesis. Cell Death Differ 27, 1952–1964 (2020).
      货号:YP0424

      Quinn, Jean A., et al. "The relationship between members of the canonical NF-kB pathway, tumour microenvironment and cancer specific survival in colorectal cancer patients." Histology and Histopathology: Cellular and Molecular Biology 35.6 (2020): 569-578.
      货号:YP0424

      • 产品图片
      • Lei, WL., Han, F., Hu, MW. et al. Protein phosphatase 6 is a key factor regulating spermatogenesis. Cell Death Differ 27, 1952–1964 (2020).
      • Western Blot analysis of 293T cells using Phospho-Tak1 (T187) Polyclonal Antibody diluted at 1:1000
      • Western blot analysis of lysates from NIH/3T3 cells treated with heat shock, using MAP3K7 (Phospho-Thr187) Antibody. The lane on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from 1)3T3 , 2) 293T, 3) HeLa cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.