IKKα (phospho Thr23) Polyclonal Antibody

    • 货号:YP0140
    • 应用:WB,IHC-p,IF(paraffin section),ELISA
    • 种属:Human,Mouse,Rat
      • 基因名称:
      • CHUK
      • 蛋白名称:
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • Human Gene Id:
      • 1147
      • Human Swiss Prot No:
      • O15111
      • Mouse Swiss Prot No:
      • Q60680
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23. AA range:15-64
      • 特异性:
      • Phospho-IKKα (T23) Polyclonal Antibody detects endogenous levels of IKKα protein only when phosphorylated at T23.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Rabbit
      • 稀释:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
      • 分子量:
      • 84654
      • 背景:
      • conserved helix-loop-helix ubiquitous kinase(CHUK) Homo sapiens This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
      • 细胞定位:
      • intracellular,nucleoplasm,cytoplasm,cytosol,IkappaB kinase complex,cytoplasmic side of plasma membrane,CD40 receptor complex,intracellular membrane-bounded organelle,
      • 组织表达:
      • Brain,Cervix carcinoma,Heart,Lymph,T-cell,
      • 产品图片
      • Western Blot analysis of various cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of KB cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IKK-alpha (Phospho-Thr23) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is blocked with the phospho peptide.