HSC 70 Polyclonal Antibody

    • 货号:YN5559
    • 应用:WB;IHC;IF
    • 种属:Human;Mouse;Rat
      • 靶点:
      • HSC70
      • 简介:
      • >>Spliceosome;>>MAPK signaling pathway;>>Protein processing in endoplasmic reticulum;>>Endocytosis;>>Longevity regulating pathway - multiple species;>>Antigen processing and presentation;>>Estrogen signaling pathway;>>Prion disease;>>Legionellosis;>>Toxoplasmosis;>>Measles;>>Lipid and atherosclerosis
      • 基因名称:
      • HSPA8
      • 蛋白名称:
      • Heat shock cognate 71 kDa protein
      • Human Gene Id:
      • 3312
      • Human Swiss Prot No:
      • P11142
      • Mouse Swiss Prot No:
      • P63017
      • 免疫原:
      • Synthesized peptide derived from the Internal region of human HSC 70. AA range: 588-638
      • 特异性:
      • The antibody detects endogenous HSC 70 protein.
      • 组成:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000.IHC-p:1:50-300.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • HSPA8;HSC70;HSP73;HSPA10;Heat shock cognate 71 kDa protein;Heat shock 70 kDa protein 8
      • 实测条带:
      • 70-72kD
      • 背景:
      • This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],
      • 功能:
      • function:Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.,induction:Constitutively synthesized.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Interacts with PACRG and TSC2. Interacts with SV40 VP1.,tissue specificity:Ubiquitous.,
      • 细胞定位:
      • Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock.
      • 组织表达:
      • Ubiquitous.
      • 产品图片
      • Western blot analysis of 1) Hela, 2) HepG2, 3) Raw, 4) Mouse Brain, 5) Rat Brain, 6) Rat Liver using HSC 70 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using HSC 70 Polyclonal Antibody.