IRE1α rabbit pAb

  • 货号:YN5428
  • 应用:WB
  • 种属:Human;Mouse;Rat
    • 靶点:
    • IRE1α
    • 简介:
    • >>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Lipid and atherosclerosis
    • 基因名称:
    • ERN1
    • 蛋白名称:
    • Serine/threonine-protein kinase/endoribonuclease IRE1
    • Human Gene Id:
    • 2081
    • Human Swiss Prot No:
    • O75460
    • Mouse Swiss Prot No:
    • Q9EQY0
    • 免疫原:
    • Purified recombinant fragment of human IRE1α (aa282-433) expressed in E. Coli.
    • 特异性:
    • IRE1α Antibody detects endogenous levels of IRE1α protein.
    • 组成:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • 来源:
    • Polyclonal, Rabbit,IgG
    • 稀释:
    • WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
    • 纯化工艺:
    • Affinity purification
    • 储存:
    • -15°C to -25°C/1 year(Do not lower than -25°C)
    • 其他名称:
    • ERN1;IRE1;Serine/threonine-protein kinase/endoribonuclease IRE1;Endoplasmic reticulum-to-nucleus signaling 1;Inositol-requiring protein 1;hIRE1p;Ire1-alpha;IRE1a
    • 分子量:
    • 105kD
    • 背景:
    • The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008],
    • 功能:
    • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains
    • 细胞定位:
    • Endoplasmic reticulum membrane ; Single-pass type I membrane protein .
    • 组织表达:
    • Ubiquitously expressed. High levels observed in pancreatic tissue.