Villin (ABT-VIL) mouse mAb
- 货号：YM6221
- 应用：IHC;IF
- 种属：Human
- 免疫原：
- Synthesized peptide derived from human Villin
- 特异性：
- This antibody detects endogenous levels of human Villin. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Monoclonal, Mouse/IgG2b, Kappa
- 稀释：
- IHC-p 1:100-500，WB 1:500-2000. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 背景：
- This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008],
- 功能：
- domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.,
- 细胞定位：
- Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .
- 组织表达：
- Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).
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- Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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- Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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- Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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- Western blot analysis of VillinAntibody at 1:1000 dilution.
- Immunohistochemical analysis of paraffin-embedded human Hepatocholangiocarcinoma Antibody was diluted at 1:200(4° overnight).
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