Actin pan (ABT-ACTN) mouse mAb

    • 货号:YM6143
    • 应用:WB; IHC;ELISA
    • 种属:Human; Mouse (predicted: Rat; Bovin; Pig; Chick)
      • 靶点:
      • Actin pan
      • 简介:
      • >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • Actin-pan
      • 蛋白名称:
      • Actin pan
      • 免疫原:
      • Synthesized peptide derived from human Actin pan AA range: 300-377
      • 特异性:
      • This antibody detects endogenous levels of Actin pan. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Mouse, Monoclonal/IgG2a, Kappa
      • 稀释:
      • IHC-p 1:200-400,WB 1:500-2000, ELISA 1:5000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 背景:
      • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • 细胞定位:
      • Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
      • 组织表达:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

      Ferritinophagy is involved in Bisphenol A-induced ferroptosis of renal tubular epithelial cells through the activation of the AMPK-mTOR-ULK1 pathway Food Chem Toxicol. 2022 May;163:112909. WB Mouse kidney tissue
      货号:YM6143

      • 产品图片
      • Human appendix tissue was stained with Anti-Actin pan (ABT-ACTN) Antibody
      • Human cardiac muscle tissue was stained with Anti-Actin pan (ABT-ACTN) Antibody
      • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Actin(ABT-ACTN) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HepG2 Lane 3: MCF7 Predicted band size: 42kDa Observed band size: 42kDa
      • Human skeletal muscle tissue was stained with Anti-Actin pan (ABT-ACTN) Antibody
      • Human stomach tissue was stained with Anti-Actin pan (ABT-ACTN) Antibody
      • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Skeletal muscle. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Western blot analysis of Actin-panAntibody at 1:1000 dilution.
      • Immunohistochemical analysis of paraffin-embedded human Salivary gland tonsils Antibody was diluted at 1:200(4° overnight).
      • Immunohistochemical analysis of paraffin-embedded human Salivary gland tonsils Antibody was diluted at 1:200(4° overnight).