BOB.1 (ABT-BOB1) mouse mAb

    • 货号:YM6111
    • 应用:IHC;WB;ELISA
    • 种属:Human; Mouse
      • 靶点:
      • BOB.1
      • 基因名称:
      • POU2AF1 OBF1
      • 蛋白名称:
      • POU domain class 2-associating factor 1 (B-cell-specific coactivator OBF-1) (BOB-1) (OCA-B) (OCT-binding factor 1)
      • Human Gene Id:
      • 5450
      • Human Swiss Prot No:
      • Q16633
      • 免疫原:
      • Synthesized peptide derived from human BOB.1 AA range: 150-256
      • 特异性:
      • This antibody detects endogenous levels of human BOB.1. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Mouse, Monoclonal/IgG2a, Kappa
      • 稀释:
      • IHC-p 1:200-400, WB 1:500-2000, ELISA 1:5000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 分子量:
      • 27kD
      • 背景:
      • disease:A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.,function:Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.,PTM:Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.,similarity:Belongs to the POU2AF1 family.,tissue specificity:B-cell specific.,
      • 功能:
      • disease:A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.,function:Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.,PTM:Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.,similarity:Belongs to the POU2AF1 family.,tissue specificity:B-cell specific.,
      • 细胞定位:
      • Nuclear, Cytoplasmic
      • 组织表达:
      • B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290).
      • 产品图片
      • Human diffuse large B-cell lymphoma tissue was stained with anti-BOB.1(ABT-BOB1) antibody.
      • Human follicular lymphoma tissue was stained with anti-BOB.1(ABT-BOB1) antibody.
      • Human tonsil tissue was stained with anti-BOB.1(ABT-BOB1) antibody.
      • Human tonsil tissue was stained with anti-BOB.1(ABT-BOB1) antibody.
      • Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-BOB.1(ABT-BOB1)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: Namalwa Predicted band size: 27kDa Observed band size: 35kDa