HMGB1 (PTR2339) Mouse mAb

    • 货号:YM4697
    • 应用:WB;ELISA;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • HMGB1
      • 简介:
      • >>Base excision repair;>>Autophagy - animal;>>Necroptosis;>>Neutrophil extracellular trap formation
      • 基因名称:
      • HMGB1 HMG1
      • 蛋白名称:
      • High mobility group protein B1 (High mobility group protein 1) (HMG-1)
      • Human Gene Id:
      • 3146
      • Human Swiss Prot No:
      • P09429
      • Mouse Swiss Prot No:
      • P63158
      • 免疫原:
      • Synthesized peptide derived from human HMGB1 AA range: 100-200
      • 特异性:
      • This antibody detects endogenous levels of HMGB1 at Human, Mouse,Rat
      • 组成:
      • PBS, pH7.4, 50% glycerol, 0.03%Proclin 300
      • 来源:
      • Mouse,monoclonal:IgG1, Kappa
      • 稀释:
      • WB 1:500-2000,ELISA 1:5000-20000
      • 纯化工艺:
      • Protein G
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • High mobility group protein B1 (High mobility group protein 1) (HMG-1)
      • 实测条带:
      • 24kDa
      • 背景:
      • high mobility group box 1(HMGB1) Homo sapiens This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],
      • 功能:
      • function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,
      • 细胞定位:
      • Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune a
      • 组织表达:
      • Ubiquitous. Expressed in platelets (PubMed:11154118).

      Doxorubicin-Loaded Platelet Decoys for Enhanced Chemoimmunotherapy Against Triple-Negative Breast Cancer in Mice Model. Yunfeng Fu WB Mouse 4T1 cell
      货号:YM4697

      • 产品图片
      • A431 whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HMGB1(PTR2339)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Predicted band size: 24kDa Observed band size: 24kDa
      • Various  whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HMGB1(PTR2339)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: A549 Lane 3: LN18 Predicted band size: 24kDa Observed band size: 24kDa
      • K562 whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-HMGB1(PTR2557)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Predicted band size: 24kDa Observed band size: 24kDa
      • MDCK whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HMGB1(PTR2339)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: MDCK Predicted band size: 24kDa Observed band size: 25kDa
      • NIH-3T3 whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HMGB1(PTR2339)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Predicted band size: 24kDa Observed band size: 26kDa
      • PC-12 whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HMGB1(PTR2339)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Predicted band size: 24kDa Observed band size: 24kDa