ERCC1 (PT0756) Mouse mAb

    • 货号:YM4081
    • 应用:WB;IHC;ELISA
    • 种属:Human
      • 靶点:
      • ERCC1
      • 简介:
      • >>Platinum drug resistance;>>Nucleotide excision repair;>>Fanconi anemia pathway
      • 基因名称:
      • ERCC1
      • 蛋白名称:
      • ERCC1
      • Human Gene Id:
      • 2067
      • Human Swiss Prot No:
      • P07992
      • 免疫原:
      • Synthesized peptide derived from human ERCC1 AA range: 1-100
      • 特异性:
      • This antibody detects endogenous levels of human ERCC1
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Mouse, Monoclonal/IgG1, Kappa
      • 稀释:
      • WB 1:500-2000,IHC 1:100-300,IF 1:100-300,ELISA 1:5000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • DNA excision repair protein ERCC-1
      • 分子量:
      • 33kD
      • 背景:
      • The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein ge
      • 功能:
      • disease:Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.,function:Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.,similarity:Belongs to the ERCC1/RAD10/SWI10 family.,subunit:Heterodimer composed of ERCC1 and XPF/ERRC4.,
      • 细胞定位:
      • [Isoform 1]: Nucleus .; [Isoform 2]: Cytoplasm . Nucleus .; [Isoform 3]: Nucleus .; [Isoform 4]: Nucleus .
      • 组织表达:
      • Cerebellum,Lung,Ovarian cancer,Uterus,
      • 产品图片
      • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-ERCC1 (PT0756) antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2 Predicted band size: 33kDa Observed band size: 33kDa