DDDDK-Tag(binds to flag sequnence) Rabbit mAb(M11)

    • 货号:YM3809
    • 应用:WB;ELISA;IF
    • 种属:Species independent
      • 纯度:
      • >95% SDS-PAGE
      • 靶点:
      • Flag-tag
      • 简介:
      • This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
      • 基因名称:
      • Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
      • 免疫原:
      • Synthetic Peptide of Flag-Tag
      • 特异性:
      • The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.
      • 组成:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • 来源:
      • Rabbit VH IgG1 and VL Kappa was expressed from 293F cells
      • 稀释:
      • WB 1:1000-5000 ELISA 1:20000-50000 IF 1:100-300
      • 纯化工艺:
      • Protein A purified
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 背景:
      • The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

      Phosphorylation of PBK at Thr9 by CDK5 correlates with invasion of prolactinomas CNS Neuroscience & Therapeutics Qiuyue Fang WB,CoIP Mouse,Human 1:5000 GH3 cell-Xenograft HEK293T cell
      货号:YM3809

      • 产品图片
      • Western Blot analysis of 293F and CHO cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:3000 dilution.
      • Western Blot analysis of flag-tag protein using primary antibody at various dilution. Secondary antibody(catalog#:RS0002) was diluted at 1:10000