DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)
- 货号：YM3808
- 应用：WB;ELISA;IP;IF;CoIP
- 种属：Species independent
- 纯度：
- >95% SDS-PAGE
- 靶点：
- Flag-tag
- 基因名称：
- Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
- 免疫原：
- Synthetic Peptide of Flag-Tag
- 特异性：
- This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i
- 组成：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 来源：
- Mouse VH IgG1 and VL Kappa was expressed from 293F cells
- 稀释：
- WB 1:10000-50000 ELISA 1:20000-50000 IP 1:50-1:200 IF 1:1000 -3000
- 纯化工艺：
- Protein A purified
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Pathogenic variants in TSC2 might cause premature ovarian insufficiency through activated mTOR induced hyperactivation of primordial follicles
货号：YM3808
Tat-hspb1 Suppresses Clear Cell Renal Cell Carcinoma (ccRCC) Growth via Lysosomal Membrane Permeabilization
货号：YM3808
circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop IF,CoIP,RIP Human /SW620, HCT116 Cells
货号：YM3808
SLC35E2 promoter mutation as a prognostic marker of esophageal squamous cell carcinoma Life Sci. 2022 May;296:120447. IF Human 1:2000
货号：YM3808
The deubiquitinase USP6 affects memory and synaptic plasticity through modulating NMDA receptor stability. PLOS BIOLOGY Plos Biol. 2019 Dec;17(12):e3000525 CoIP Human,Mouse 1:3000 Brain HEK293T cell
货号：YM3808
miR-375 is involved in Hippo pathway by targeting YAP1/TEAD4-CTGF axis in gastric carcinogenesis. Cell Death & Disease 2018 Jan 24 WB Human AGS cell
货号：YM3808
Salmonella Effector SpvB Inhibits NF-κB Activity via KEAP1-Mediated Downregulation of IKKβ. Frontiers in Cellular and Infection Microbiology Front Cell Infect Mi. 2021 Mar;11:207 WB Human HeLa cells，293T cells
货号：YM3808
Identification of the internal ribosome entry sites in the 5'‑untranslated region of the c‑fos gene. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2021 Apr;47(4):1-1 IP Human 1: 5000 293T cell
货号：YM3808
Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2018 Apr;41(4):2169-2176 IF Human 1:1000 HepG2.2.15 cell
货号：YM3808
CMTM7 inhibits breast cancer progression by regulating Wnt/β-catenin signaling BREAST CANCER RESEARCH Yue Yu CoIP Human MCF-7 cell
货号：YM3808
Microtubule-associated protein 4 promotes epithelial mesenchymal transition in hepatocellular cancer cells via regulating GSK3β/β-catenin pathway Heliyon Pingping Hu WB Human MHCC97-L cell
货号：YM3808
Mutant p53 activates hnRNPA2B1-AGAP1-mediated exosome formation to promote esophageal squamous cell carcinoma progression CANCER LETTERS Zhihua Liu WB Human KYSE510 cell
货号：YM3808
RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Zhihua Liu WB,IP Human 1:5000 HEK 293 T cell,KYSE30 cell,KYSE450 cell
货号：YM3808
Cystathionine β-synthase (CBS) suppresses NLRP3 inflammasome activation via redox regulation in microglia. Dr. Lifang Hu WB Mouse 1:1000 BV2 cell
货号：YM3808
Anoikis resistance and immune escape mediated by Epstein-Barr virus-encoded latent membrane protein 1-induced stabilization of PGC-1α promotes invasion and metastasis of nasopharyngeal carcinoma. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Xiangjian Luo CoIP Human 293T cell
货号：YM3808
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- Antibody-FAQs
- 产品图片
- RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Zhihua Liu WB,IP Human 1:5000 HEK 293 T cell,KYSE30 cell,KYSE450 cell
- Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
- Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody(catalog#:RS0001) was diluted at 1:10000
- 1) Input: 293T transfected N-flag protein Lysate 2) IP product: IP dilute 1: 200, 293T transfected N-flag protein Lysate Western blot analysis: primary antibody : 1:10000 Secondary antibody: Goat anti-Mouse IgG(RS0001), 1: 5000
- Immunofluorescence analysis of 293T transfected FLAG and GFP tag protein. 1, FLAG primary Antibody(red YM3808) was diluted at 1:1000(4°C overnight), GFP primary Antibody(green YN5582) was diluted at 1:1000(4°C overnight),
