β II tubulin Monoclonal Antibody(Mix)

    • 货号:YM3151
    • 应用:WB;IHC;IF;IP
    • 种属:Mouse;Rat;(H)
      • 靶点:
      • Tubulin β II
      • 简介:
      • >>Phagosome;>>Gap junction;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection
      • 基因名称:
      • TUBB2A
      • 蛋白名称:
      • Tubulin beta-2A chain
      • Human Gene Id:
      • 7280
      • Human Swiss Prot No:
      • Q13885
      • Mouse Swiss Prot No:
      • Q7TMM9
      • 免疫原:
      • Synthetic Peptide of β II tubulin
      • 特异性:
      • The antibody detects endogenous β II tubulin protein.
      • 组成:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • WB 1:50000-100000 IP:1:200 IHC 1:50-300. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • Tubulin beta-2A chain
      • 实测条带:
      • 50kD
      • 背景:
      • Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015],
      • 功能:
      • function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,
      • 细胞定位:
      • Cytoplasm, cytoskeleton .
      • 组织表达:
      • High expression in brain, where it represents 30% of all beta-tubulins.
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,β II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,β II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,β II tubulin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, diluted at 1:100000.
      • 1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1: 200 Western blot analysis: primary antibody : TDY191 1: 10000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1: 5000