VSV-G-Tag Monoclonal Antibody(8D6)
- 货号：YM3006
- 应用：WB;IP;IF
- 种属：Species independent
- 免疫原：
- Synthetic Peptide of VSV-G-Tag
- 特异性：
- The antibody detects C-terminal, internal, and N-terminal VSV-G fusion proteins.
- 组成：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 稀释：
- WB 1:5000 IP: 1:200 IF 1:1000
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.
- Western blot analysis of VSV-G-TAG protein, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920 was diluted at 1:10000, 37° 1hour.
- 1ug VSV-G fusion protein+ Primary antibody dilution at
1) 1:5000
2) 1:10000
- IF analysis of 293T cells transfected with a VSV-G-tagged protein,1:2000 dilution (blue DAPI, red anti-VSV-G)
- IP antibody use:5ug VSV-G Mouse IgG1 per ml Lysate,WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with VSV-G-tag fusion protein
3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose)
4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)
6、IP (transfected 293+Protein G)
7、Recombinant protein (E.coli)
