Rab 10 Monoclonal Antibody
- 货号：YM0544
- 应用：WB;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Endocytosis;>>AMPK signaling pathway
- 蛋白名称：
- Ras-related protein Rab-10
- 免疫原：
- Purified recombinant fragment of human Rab 10 expressed in E. Coli.
- 特异性：
- Rab 10 Monoclonal Antibody detects endogenous levels of Rab 10 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RAB10;Ras-related protein Rab-10
- 背景：
- RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009],
- 功能：
- function:May be involved in vesicular trafficking and neurotransmitter release.,similarity:Belongs to the small GTPase superfamily. Rab family.,
- 细胞定位：
- Cytoplasmic vesicle membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Recycling endosome membrane . Cytoplasmic vesicle, phagosome membrane . Cytoplasm, cytoskeleton, cilium basal body . Endoplasmic reticulum membrane . Cytoplasm, perinuclear region . Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium when phosphorylated by LRRK2 on Thr-73 (PubMed:20576682, PubMed:30398148). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280). .
- 组织表达：
- Expressed in the hippocampus (PubMed:29562525). Expressed in neutrophils (at protein level) (PubMed:29127255). Expressed in the testis (at protein level) (PubMed:28067790).
- Western Blot analysis using Rab 10 Monoclonal Antibody against HeLa (1) and NIH/3T3 (2) cell lysate.
- Immunofluorescence analysis of LOVO cells using Rab 10 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
