eNOS Monoclonal Antibody

    • 货号:YM0480
    • 应用:IHC;IF;ELISA
    • 种属:Human
      • 靶点:
      • NOS3/eNOS
      • 简介:
      • >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>PI3K-Akt signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Platelet activation;>>Estrogen signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • NOS3
      • 蛋白名称:
      • Nitric oxide synthase, endothelial
      • Human Gene Id:
      • 4846
      • Human Swiss Prot No:
      • P29474
      • Mouse Swiss Prot No:
      • P70313
      • 免疫原:
      • Purified recombinant fragment of human NOS3 expressed in E. Coli.
      • 特异性:
      • NOS3 Monoclonal Antibody detects endogenous levels of NOS3 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
      • 纯化工艺:
      • Affinity purification
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • NOS3;Nitric oxide synthase; endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII
      • 实测条带:
      • 130-140kD
      • 背景:
      • Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
      • 功能:
      • catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like
      • 细胞定位:
      • Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      • 组织表达:
      • Platelets, placenta, liver and kidney.
      • 产品图片
      • Immunohistochemistry analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues with DAB staining using NOS3 Monoclonal Antibody.