Cyclin B1 Monoclonal Antibody

    • 货号:YM0175
    • 应用:WB;IP;IF;FCM;ELISA
    • 种属:Human;Rat
      • 靶点:
      • Cyclin B1
      • 简介:
      • >>FoxO signaling pathway;>>Cell cycle;>>Oocyte meiosis;>>p53 signaling pathway;>>Cellular senescence;>>Progesterone-mediated oocyte maturation;>>Human immunodeficiency virus 1 infection
      • 基因名称:
      • CCNB1
      • 蛋白名称:
      • G2/mitotic-specific cyclin-B1
      • Human Gene Id:
      • 891
      • Human Swiss Prot No:
      • P14635
      • Mouse Swiss Prot No:
      • P24860
      • 免疫原:
      • Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.
      • 特异性:
      • Cyclin B1 Monoclonal Antibody detects endogenous levels of Cyclin B1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • Affinity purification
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CCNB1;CCNB;G2/mitotic-specific cyclin-B1
      • 分子量:
      • 48kD
      • 背景:
      • The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],
      • 功能:
      • developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.,
      • 细胞定位:
      • Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
      • 组织表达:
      • Breast adenocarcinoma,Lung,Placenta,

      Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression. JOURNAL OF PROTEOME RESEARCH J Proteome Res. 2021;20(5):2329–2339 WB,IP Human 1 : 500 HeLa cell
      货号:YM0175

      • 产品图片
      • Western Blot analysis using Cyclin B1 Monoclonal Antibody against HeLa (1) and PC-12 (2) cell lysate.
      • Immunofluorescence analysis of Hela cells using Cyclin B1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
      • Flow cytometric analysis of Hela cells using Cyclin B1 Monoclonal Antibody (green) and negative control (purple).