IKKα (phospho Thr23) Polyclonal Antibody

    • Catalog No.:YP0140
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • IKKα
      • Fields:
      • >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>FoxO signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Adipocytokine signaling pathway;>>Alcoholic liver disease;>>Alzheimer disease;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Chagas disease;>>Toxoplasmosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human pap
      • Gene Name:
      • CHUK
      • Protein Name:
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha
      • Human Gene Id:
      • 1147
      • Human Swiss Prot No:
      • O15111
      • Mouse Swiss Prot No:
      • Q60680
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23. AA range:15-64
      • Specificity:
      • Phospho-IKKα (T23) Polyclonal Antibody detects endogenous levels of IKKα protein only when phosphorylated at T23.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CHUK;IKKA;TCF16;Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B
      • Molecular Weight(Da):
      • 85kD
      • Background:
      • This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK
      • Subcellular Location:
      • Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.
      • Expression:
      • Widely expressed.
      • Products Images
      • Western Blot analysis of various cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of KB cells using Phospho-IKKα (T23) Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IKK-alpha (Phospho-Thr23) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is blocked with the phospho peptide.